The effect of temperature conditions during growth on the transformation frequency of Coccomyxa subellipsoidea C-169 obtained by electroporation

Kinga Kania; Anna Levytska; Anna Drożak; Borkowski Andrzej; Działak Paweł; Maksymilian Zienkiewicz

Biochemistry and Biophysics Reports (Jul 2022)

The effect of temperature conditions during growth on the transformation frequency of Coccomyxa subellipsoidea C-169 obtained by electroporation

Kinga Kania,
Anna Levytska,
Anna Drożak,
Borkowski Andrzej,
Działak Paweł,
Maksymilian Zienkiewicz

Affiliations

Kinga Kania: Department of Molecular Plant Physiology, University of Warsaw, Warsaw, Poland
Anna Levytska: Department of Molecular Plant Physiology, University of Warsaw, Warsaw, Poland
Anna Drożak: Department of Molecular Plant Physiology, University of Warsaw, Warsaw, Poland
Borkowski Andrzej: Faculty of Geology, Geophysics and Environmental Protection, AGH University of Science and Technology, Krakow, Poland
Działak Paweł: Faculty of Geology, Geophysics and Environmental Protection, AGH University of Science and Technology, Krakow, Poland
Maksymilian Zienkiewicz: Department of Molecular Plant Physiology, University of Warsaw, Warsaw, Poland; Corresponding author.

Journal volume & issue: Vol. 30
p. 101220

Abstract

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In this study, we have shown that transformation efficiency of Coccomyxa subellipsoidea C-169 obtained by electroporation can be significantly increased by either supra- or sub-optimal growth temperatures.

Published in Biochemistry and Biophysics Reports

ISSN: 2405-5808 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General); Science: Chemistry: Organic chemistry: Biochemistry
Website: http://www.journals.elsevier.com/biochemistry-and-biophysics-reports/

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