Hemijska Industrija (Jan 2012)

Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase

  • Mandić Ljuba M.,
  • Mitić Violeta D.,
  • Ilić Marija D.,
  • Stankov-Jovanović Vesna P.,
  • Nikolić-Mandić Snežana D.

DOI
https://doi.org/10.2298/HEMIND120128032S
Journal volume & issue
Vol. 66, no. 5
pp. 677 – 684

Abstract

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Propranolol, a widely used beta-blocker, inhibits the reaction of enzyme cholinesterase hydrolysis. Measurements of the hydrolysis rate differences between and inhibited reactions, allows the development of a kinetic method for its determination. Both systems, enzyme-substrate-chromogen and enzyme-substrate-chromogen-inhibitor, were characterized through biochemical kinetic parameters (KM = 0.326-0.330 mmol/L; Vmax = 40-42.99 μmol/Lmin), inhibition type was recognized as competitive, and inhibition constant, Ki, was determined to be 22.60 μmol/L. The detection and quantification limits were calculated as 0.004 and 0.0136 μmol/L, respectively. Accuracy and precision of proposed methods were tested. Proposed method is characterized with good sensitivity, selectivity, simplicity and rapidity, thus it is convenient for clinical applications.

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