Functional GUS assay of GRAS transcription factor from Medicago truncatula

Abstract

GRAS gene family encodes transcription factors (TFs) which plays a vital role in different processes of plant growth and development of stems and roots, phytohrome-A signal transduction (PAT), gibberellic acid signal transduction (GA), plant response to biotic and abiotic stress conditions and disease resistance. They could be induced by salt and drought stress and in response to pathogen attack. In this research we investigated the expression pattern of the gene encoding MtGRAS TF (Medtr2g026250) by functional GUS assay in the model legume species Medicago truncatula. Transcriptional reporter lines carrying the promoter region of the gene encoding MtGRAS TF fused to GUS and GFP reporter genes were obtained via Agrobacterium-transformation of suspension cultures. The activity of the GUS reporter gene was detected in various parts and tissues of T1 generation of transformed plants, as well as under salinity stress.

Keywords

• gras transcription factor
• medicago truncatula
• gus assay
• salinity stress