Hypertension in Pregnancy (Jan 2022)

Total Th1/Th2 cytokines profile from peripheral blood lymphocytes in normal pregnancy and preeclampsia syndrome

  • Iara dos Santos Fagundes,
  • Eduardo Pletsch Brendler,
  • Isadora Nunes Erthal,
  • Raquel Jaqueline Eder Ribeiro,
  • Rafaela Siviero Caron-Lienert,
  • Denise Cantarelli Machado,
  • Bartira Ercília Pinheiro da Costa,
  • Carlos Eduardo Poli-de-Figueiredo

DOI
https://doi.org/10.1080/10641955.2021.2008424
Journal volume & issue
Vol. 41, no. 1
pp. 15 – 22

Abstract

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To evaluate total Th1/Th2 cytokines in CD3+ cells (immunocompetent T-lymphocytes) and peripheral blood lymphocytes, mostly CD4+ (T helper cells) and CD8+ (T-cytotoxic cells) subpopulations in preeclampsia. Total blood leukocytes and lymphocytes counts, percent cells: CD3+, INF-g+/CD3+, IL-4+/CD3+, and IL-10+/CD3+, CD4+/CD8+ were determined by flow-cytometry. Preeclampsia (n= 26) and normal pregnancy (n= 25) participants were age and gestational age matched. CD4+ lymphocytes count was higher in preeclampsia, compared with normal pregnancy (43.6 ± 5.8 vs 37.6 ± 5.6%; P< 0.001). CD3+ cells Th1/Th2 shift was not detected in preeclampsia, yet may be present in other cell types, such as CD4+ and CD3 – lymphocytes.

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