Agronomy Science (Dec 2012)
The effect of Fe-deficiency on antioxidant enzymes’ activity and lipid peroxidation in wheat leaves
Abstract
Studying the physiological role of nutrient elements has an unraveling capacity in understanding plant’s behavioral pattern in order to acquire a high and stable yield. For this purpose, the durum wheat variety, P1252, was selected and planted in hydroponic way. To investigate the effects of Fe deficiency, the element was eliminated from media solutions. Results showed that the lack of Fe affected superoxide dismutase (SOD), guaicol peroxidase (GPX) and catalase (CAT) activities significantly. Meanwhile, ascorbate peroxidase (APX) was the only antioxidant enzyme not shows any significant with control. The SOD/(APX + GPX + CAT) ratio as an index of assessing the balance between hydrogen peroxide (H2O2)-producing and H2O2-scavenging enzymes increased leading to the accumulation of H2O2 in cell. The elevation of SOD/APX + GPX + CAT ratio and H2O2 accumulation indicates the occurrence of oxidative stress in the leave cells in the element-deletion conditions. Other oxidative stress indices, cell death as well as malondialdehyde (MDA) did not show any significant change in the absence of Fe. The reason is assumed to be the non-occurrence of Haber-Weiss reaction in Fe absence so that hydroxyl, a very dangerous radical, is produced, leading to increased damage to cell bio-molecules and apoptosis subsequently.
