Role of ATP5B in colorectal cancer

Abstract

Abstract Objective To explore the role and possible regulatory mechanisms of ATP5B in colorectal cancer. Methods Online databases were used to analyze expression of ATP5B and prognosis in patients with l colorectal cancer. The data of TCGA database were analyzed by Gene Set Enrichment Analysis. Co‐expression network analysis of ATP5B was analyzed by coexpedia. Colorectal cancer cell (SW480 and DLD1) were transfected with small interfering RNA and validated by real‐time fluorescence quantitative PCR. Cell proliferation ability was detected by CCK8 experiment, cell migration and invasion ability were detected by scratch experiment and Transwell experiment. Results ATP5B is highly expressed in colorectal cancer tissue. Up‐regulation of ATP5B is associated with poor prognosis of colorectal cancer patients. ATP5B may regulate the cell junction pathway and the PI3K‐AKT‐mTOR signaling pathway. ATP5B expression is positively correlated with the proliferation, migration, and invasion of colorectal cancer cells. Conclusions ATP5B plays an important role in the tumorigenesis and metastasis of colorectal cancer.

Keywords

• ATP5B
• colorectal cancer
• proliferation
• migration
• invasion