Histomorphometric and histologic evaluation of the effects of leukocyte platelet-rich fibrin (L-PRF) and nano-hydroxyapatite (nHA) on bone regeneration in rabbits

Abstract

Introduction: The study aimed to assess the histologic and histomorphometric effects of leukocyte platelet-rich fibrin (L-PRF) and nano-hydroxyapatite (nHA) on the regeneration of calvarial bone defects in rabbits.Methods: Four defects were created in the calvaria bone of 14 New Zealand rabbits and filled with L-PRF clot, nHA, or a combination of L-PRF and nHA. The fourth defect remained unfilled to serve as the control group. The rabbits were sacrificed either at 4 or at 8 weeks, and the specimens were evaluated for the type and degree of inflammation, foreign body reaction, new bone formation, and residual biomaterial particles.Results: The histomorphometric analysis revealed that L-PRF significantly enhanced osteogenesis (P0.05). The results of the histologic analysis showed that the frequency of central bone regeneration significantly increased in prolonged periods (P0.05).Conclusion: The results showed that L-PRF was a suitable option for the induction of bone regeneration with fewer remnant bodies. However, future clinical studies are required to assess the efficacy of these biomaterials in the clinical setting. (J Dent Mater Tech 2023;12(1): 43-50)

Keywords

• bone regeneration
• leukocyte platelet-rich fibrin
• nano-hydroxyapatite