Generation of an induced pluripotent stem cell line (ZJUi007-A) from a 11-year-old patient of Fabry disease

Yuqing Zhu; Li Zhang; Gang Wang; Jing Zhao; Xiaolin Hou; Hangdi Wu; Yuming Xu; Jianhua Mao; Zhihong Liu; Jin Zhang

Stem Cell Research (Aug 2021)

Generation of an induced pluripotent stem cell line (ZJUi007-A) from a 11-year-old patient of Fabry disease

Yuqing Zhu,
Li Zhang,
Gang Wang,
Jing Zhao,
Xiaolin Hou,
Hangdi Wu,
Yuming Xu,
Jianhua Mao,
Zhihong Liu,
Jin Zhang

Affiliations

Yuqing Zhu: Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China
Li Zhang: Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China
Gang Wang: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210093, China
Jing Zhao: Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China
Xiaolin Hou: Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China
Hangdi Wu: Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China
Yuming Xu: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Jianhua Mao: Department of Nephrology, The Children Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China
Zhihong Liu: Zhejiang Laboratory for Systems and Precision Medicine, Zhejiang University Medical Center, Hangzhou 311121, China; National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210093, China
Jin Zhang: Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China; Zhejiang Laboratory for Systems and Precision Medicine, Zhejiang University Medical Center, Hangzhou 311121, China; Institute of Hematology, Zhejiang University, Hangzhou, Zhejiang 310058, China; Corresponding author.

Journal volume & issue: Vol. 55
p. 102475

Abstract

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PBMCs were collected from a patient with a novel GLA gene mutation (c.140G > A) which contributed to Fabry disease. Subsequently, an induced pluripotent stem cell (iPSC) line was derived using an episomal reprogramming method that transfer the reprogramming plasmids expressing OCT3/4, SOX2, KLF4, LIN28 and L-MYC into the PBMCs. The expected mutation in the iPSC line was confirmed by Sanger sequencing, while the pluripotency status was validated by immunofluorescence assay and flow cytometry for pluripotency markers, as well as teratoma formation.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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