Marine Prostanoids with Cytotoxic Activity from Octocoral <i>Clavularia</i> spp.
Ming-Ya Cheng,
I-Chi Hsu,
Shi-Ying Huang,
Ya-Ting Chuang,
Tzi-Yi Ke,
Hsueh-Wei Chang,
Tian-Huei Chu,
Ching-Yeu Chen,
Yuan-Bin Cheng
Affiliations
Ming-Ya Cheng
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804201, Taiwan
I-Chi Hsu
Division of Pharmacy, Zuoying Armed Forces General Hospital, Kaohsiung 813204, Taiwan
Shi-Ying Huang
College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China
Ya-Ting Chuang
PhD Program in Life Sciences, Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung 807378, Taiwan
Tzi-Yi Ke
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804201, Taiwan
Hsueh-Wei Chang
PhD Program in Life Sciences, Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung 807378, Taiwan
Tian-Huei Chu
Medical Laboratory, Medical Education and Research Center, Kaohsiung Armed Forces General Hospital, Kaohsiung 802301, Taiwan
Ching-Yeu Chen
Department of Physical Therapy, Tzu-Hui Institute of Technology, Pingtung 926001, Taiwan
Yuan-Bin Cheng
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804201, Taiwan
Octocoral of the genus Clavularia is a kind of marine invertebrate possessing abundant cytotoxic secondary metabolites, such as prostanoids and dolabellanes. In our continuous natural product study of C. spp., two previously undescribed prostanoids [clavulone I-15-one (1) and 12-O-deacetylclavulone I (2)] and eleven known analogs (3–13) were identified. The structures of these new compounds were elucidated based on analysis of their 1D and 2D NMR, HRESIMS, and IR data. Additionally, all tested prostanoids (1 and 3–13) showed potent cytotoxic activities against the human oral cancer cell line (Ca9-22). The major compound 3 showed cytotoxic activity against the Ca9-22 cells with the IC50 value of 2.11 ± 0.03 μg/mL, which echoes the cytotoxic effect of the coral extract. In addition, in silico tools were used to predict the possible effects of isolated compounds on human tumor cell lines and nitric oxide production, as well as the pharmacological potentials.
