Influence of silicon oxide (SiO₂) on the adaptation of microplants of roses (<i>Rose L.</i>) cv. Reine Sammut

Abstract

The research is devoted to the optimization of the method of adaptation of Reine Sammut microplants of roses using silicon oxide (SiO2) by spraying and watering. For the experiment, there have been used standard microplants obtained by the method of clonal micropropagation, corresponding to GOST 29105.1-91-29105.3-91. Before planting for adaptation, the microplants were cleared from the lower leaves, the roots were washed from the agar nutrient medium in a decimolar solution of potassium permanganate and trimmed to 15-20 mm. Adaptation was carried out in micro-greenhouses on a peat nutrient substrate, made in accordance with TU 20.12.80 001 41790563 2020, in a light room (humidity 36 %, temperature 23...25 ° C, light period duration 16 hours, illumination 8000 Lx). In order to disinfect the substrate, the biofungicide "Trichoderma veride" (1.5 ml/l) was used. Microplants after planting for adaptation were shed and sprinkled in accordance with the experiment options: 1) distilled water (control); 2) 0.01% SiO2 solution; 3) 0.005% SiO2 solution; 4) 0.0025% SiO2 solution. With prolonged ventilation of micro-greenhouses, 28 % of plants in the control variant showed drying of the leaves, which did not occur when using silicon oxide solutions. According to visual diagnostics, the most attractive plant species was when watered and sprayed with 0.01% silica. This concentration of silicon oxide during plant adaptation compared to the control contributed to a significant increase in plant height and shoot length by 50.0 mm and 42.6 mm, respectively (LSD05 = 36.1 and 28.3), and a tendency to increase the number of leaves by 1. 6 pcs., shoot weight 2.0 and roots 1.8 times.

Keywords

• clonal micropropagation
• microplant
• adaptation
• morphometric parameters
• aerial part
• root system