Effect of a Synbiotic Mix on Intestinal Structural Changes, and <i>Salmonella</i> Typhimurium and <i>Clostridium Perfringens</i> Colonization in Broiler Chickens
Zuamí Villagrán-de la Mora,
Karla Nuño,
Olga Vázquez-Paulino,
Hugo Avalos,
Javier Castro-Rosas,
Carlos Gómez-Aldapa,
Carlos Angulo,
Felipe Ascencio,
Angélica Villarruel-López
Affiliations
Zuamí Villagrán-de la Mora
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur 23096 La Paz, BCS, Mexico
Karla Nuño
Departamento de Ciencias Biomédicas, Centro Universitario de Tonalá, Universidad de Guadalajara, Nuevo Perif. Ote. 555, Ejido San José, Tateposco 45425 Tonalá, Jalisco, Mexico
Olga Vázquez-Paulino
Departamento de Farmacobiología, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Gral. Marcelino García Barragán 1421, Olímpica 44430, Guadalajara, Jalisco, Mexico
Hugo Avalos
Departamento de Farmacobiología, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Gral. Marcelino García Barragán 1421, Olímpica 44430, Guadalajara, Jalisco, Mexico
Javier Castro-Rosas
Área Académica de Química, Instituto de Ciencias Básicas e Ingeniería, Ciudad del Conocimiento, Universidad Autónoma del Estado de Hidalgo, Abasolo 600, Centro 42000, Pachuca de Soto, Hidalgo, Mexico
Carlos Gómez-Aldapa
Área Académica de Química, Instituto de Ciencias Básicas e Ingeniería, Ciudad del Conocimiento, Universidad Autónoma del Estado de Hidalgo, Abasolo 600, Centro 42000, Pachuca de Soto, Hidalgo, Mexico
Carlos Angulo
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur 23096 La Paz, BCS, Mexico
Felipe Ascencio
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur 23096 La Paz, BCS, Mexico
Angélica Villarruel-López
Departamento de Farmacobiología, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Gral. Marcelino García Barragán 1421, Olímpica 44430, Guadalajara, Jalisco, Mexico
Synbiotics can prevent gastrointestinal infections in broilers. This work studies the effect of a Synbiotic on broilers. One-day-old male broilers were divided into groups: Control; Synbiotic; Synbiotic + S. Typhimurium; Synbiotic + C. perfringens; Synbiotic + S. Typhimurium + C. perfringens; S. Typhimurium; C. perfringens; and S. Typhimurium + C. perfringens. Histopathological analysis revealed that the Synbiotic promoted longer villi, less deep crypts, and better villi-crypt ratio. Broilers treated with the Synbiotic, infected with pathogens or not, had healthier mucosa. In groups infected with pathogens, the frequency and intensity of histopathologic lesions were lessened often in groups treated with the Synbiotic. The Synbiotic group had higher lactic acid bacteria counts than the Control group on day 39, and the isolation frequency of S. Typhimurium was lower (p < 0.05) in the Synbiotic-treated groups. On day 18, mucosa, villi, villi-crypt ratio, crypt, and feed intake were influenced by Enterobacteriaceae. However, on day 39 (end of the trial), those parameters were influenced by lactic acid bacteria. The Synbiotic influenced morphological modifications in the duodenal mucosa, which in turn gave the broilers the ability to resist infections caused by S. Typhimurium and C. perfringens, by inhibiting their growth and decreasing the intensity and frequency of histopathological injuries.