Histopathological assessment of wound healing after using adipose-tissue derived mesenchymal stem cells with Tragacanth gum hydrogel and human amniotic membrane as dressing

Abstract

Wound healing and finding a solution for fast healing are among of the major issues of today’s world. This study aimed to assess the effect of Tragacanth gum hydrogel as a three-dimensional scaffold of MSCs along with a wound dressing of human amniotic membrane in the healing of full-thickness skin wounds in rat. In this study, 54 Albino female rats (150 g) were divided into control, hydrogel, and hydrogel+stem cell groups. Under general anesthesia, two bilateral full-thickness wounds were created on the dorsal area by a 9.8-millimeter biopsy punch. Rats were euthanized on days 3, 10, and 21 for histopathology and cell tracking by PCR evaluation of tissue samples. The histopathological results showed that no significant difference was seen on days 3 and 21, and there were significant differences only on day 10. In terms of epithelialization, the treatment groups were significantly different from the control group hydrogel+MSCs had a statistically significant difference with the control group in terms of granulation tissue formation. Cell tracking results with PCR on days 3, 10, and 21 in the hydrogel+MSCs group showed that MSCs were found only on day 3. The results of the present study showed that the use of stem cells together with the Tragacanth gum hydrogel as a scaffold and the use of human amniotic membrane as a dressing can cause fast healing of full-thickness wounds.

Keywords

• amniotic membrane
• mscs
• rats
• scaffold
• wound healing