Isolation and Characterization of 20 Polymorphic Microsatellite Markers for <i>Juglans mandshurica</i> (Juglandaceae)

Abstract

Premise of the study: Fifty microsatellite loci were developed for the endangered species Juglans mandshurica to investigate its genetic diversity and population structure. Methods and Results: In all, 50 microsatellite markers were isolated from J. mandshurica, using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. Twenty of these polymorphic markers were assessed in samples collected from 98 individuals among five populations in northeastern China. Across all of the J. mandshurica samples, the number of alleles per locus ranged from one to 17. Conclusions: These new microsatellite loci will be useful for conservation genetics studies of J. mandshurica.

Keywords

• genetic conservation
• Juglandaceae
• Juglans mandshurica
• microsatellite marker
• simple sequence repeat