Nature Communications (Nov 2017)

Nanopore electric snapshots of an RNA tertiary folding pathway

  • Xinyue Zhang,
  • Dong Zhang,
  • Chenhan Zhao,
  • Kai Tian,
  • Ruicheng Shi,
  • Xiao Du,
  • Andrew J. Burcke,
  • Jing Wang,
  • Shi-Jie Chen,
  • Li-Qun Gu

DOI
https://doi.org/10.1038/s41467-017-01588-z
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 11

Abstract

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While RNA folding is critical for its function, study of this process is challenging. Here, the authors combine nanopore single-molecule manipulation with theoretical analysis to follow the folding of an RNA pseudoknot, monitoring the intermediate states and the kinetics of their interconversion.