Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6

Patrick Guye; Mohammad R. Ebrahimkhani; Nathan Kipniss; Jeremy J. Velazquez; Eldi Schoenfeld; Samira Kiani; Linda G. Griffith; Ron Weiss

doi:10.1038/ncomms10243

Nature Communications (Jan 2016)

Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6

Patrick Guye,
Mohammad R. Ebrahimkhani,
Nathan Kipniss,
Jeremy J. Velazquez,
Eldi Schoenfeld,
Samira Kiani,
Linda G. Griffith,
Ron Weiss

Affiliations

Patrick Guye: Department of Biological Engineering, Massachusetts Institute of Technology (MIT)
Mohammad R. Ebrahimkhani: Department of Biological Engineering, Massachusetts Institute of Technology (MIT)
Nathan Kipniss: Department of Biological Engineering, Massachusetts Institute of Technology (MIT)
Jeremy J. Velazquez: Department of Biological Engineering, Massachusetts Institute of Technology (MIT)
Eldi Schoenfeld: Department of Biological Engineering, Massachusetts Institute of Technology (MIT)
Samira Kiani: Department of Biological Engineering, Massachusetts Institute of Technology (MIT)
Linda G. Griffith: Department of Biological Engineering, Massachusetts Institute of Technology (MIT)
Ron Weiss: Department of Biological Engineering, Massachusetts Institute of Technology (MIT)

DOI: https://doi.org/10.1038/ncomms10243
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

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There has been limited success in generating tissues from human induced pluripotent stem cells (hiPSCs). Here, the authors genetically engineer expression of the transcription factor Gata6 in a single isogenic hiPSC population resulting in complex tissue structures that exhibit liver bud-like properties.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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