PPARG expression patterns and correlations in obesity

Abstract

Background: Obesity is a result of excess fat storage in tissues and may lead to local insulin resistance and inflammation. Obesity is a multifactorial event and may include dysregulation of certain genes, such as peroxisome proliferator activated receptor gamma (PPARG). PPARG plays a key role in adipogenesis and adipocyte gene expression. Aim: Our study aimed to determine the regulatory role of PPARG in obesity. Methods: Serum samples from obese, non-obese(controls), diabetic obese and diabetic individuals were collected, and total RNA was extracted. RNA was reversed to cDNA and real time PCR technology was used to quantify differential PPARG gene expression in serum of obese patients and in diabetic obese patients (BMI > 30 kg/m2) versus non-obese and diabetic controls. Anthropometric parameters were recorded, and laboratory measurements were performed for each subject. Demographic and biochemical characteristics were studied for their correlation to PPARG mRNA in each group. The data were analyzed using an ANOVA analysis (p < 0.05). Results: PPARG mRNA expression is most abundant in serum of obese patients both diabetic and non-diabetic. However, differential level of PPARG mRNA expression was seen in diabetic patients without obesity being only 40% of group subjects showed overexpressed PPARG in serum. A statistically significant differences were observed for mean TGs, TC, LDLc and HDLc levels among groups. There was a strong positive correlation between PPARG mRNA and the body mass index, waist circumference (WC) and waist–hip ratio (WHR) of obese patients.

Keywords

• Obesity
• PPARG
• BMI
• WC