Food and Agricultural Immunology (Jan 2018)

Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip

  • Liqiang Liu,
  • Liguang Xu,
  • Steven Suryoprabowo,
  • Shanshan Song,
  • Hua Kuang

DOI
https://doi.org/10.1080/09540105.2017.1376040
Journal volume & issue
Vol. 29, no. 1
pp. 358 – 368

Abstract

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In this paper, we describe the development of a rapid, simple, specific, and sensitive immunochromatographic strip test based on an antibody–antigen reaction for detection of tulathromycin (TULA) in samples of 0.01 M PBS pH 7.4, pure milk and honey. The monoclonal antibodies specifically recognized the corresponding antigens produced in our laboratory. Under optimized conditions, the cut-off limits of the test strips for TULA in 0.01 M PBS pH 7.4 was found to be 2.5 ng/mL. Meanwhile, with simple preparation for detection in pure milk and honey samples, the cut-off limits for TULA were found to be 5 and 10 ng/mL, respectively. Each test can be evaluated in 5 min. In summary, this lateral-flow device provides a sensitive, effective, specific, and rapid method for detection of TULA residues in food matrix.

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