Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase [version 1; peer review: 2 approved, 1 approved with reservations]

Ulrich Dobramysl; Tom Beneke; Jeremy Charles Mottram; Carolina Moura Costa Catta-Preta; Richard Wheeler; Eva Gluenz

Wellcome Open Research (Dec 2022)

Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase [version 1; peer review: 2 approved, 1 approved with reservations]

Ulrich Dobramysl,
Tom Beneke,
Jeremy Charles Mottram,
Carolina Moura Costa Catta-Preta,
Richard Wheeler,
Eva Gluenz

Affiliations

Ulrich Dobramysl: ORCiD; Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, UK
Tom Beneke: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
Jeremy Charles Mottram: ORCiD; York Biomedical Research Institute, Department of Biology, University of York, York, UK
Carolina Moura Costa Catta-Preta: York Biomedical Research Institute, Department of Biology, University of York, York, UK
Richard Wheeler: ORCiD; Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, UK
Eva Gluenz: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK

Journal volume & issue: Vol. 7

Abstract

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We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.

Published in Wellcome Open Research

ISSN: 2398-502X (Online)
Publisher: Wellcome
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://wellcomeopenresearch.org/

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Abstract

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