Cell Biological and Antibacterial Evaluation of a New Approach to Zirconia Implant Surfaces Modified with MTA
Beatriz Ferreira Fernandes,
Neusa Silva,
Mariana Brito Da Cruz,
Gonçalo Garret,
Óscar Carvalho,
Filipe Silva,
António Mata,
Helena Francisco,
Joana Faria Marques
Affiliations
Beatriz Ferreira Fernandes
Oral Biology and Biochemistry Research Group—Unidade de Investigação em Ciências Orais e Biomédicas (UICOB), Faculdade de Medicina Dentária, Universidade de Lisboa, 1600-277 Lisboa, Portugal
Neusa Silva
Oral Biology and Biochemistry Research Group—Unidade de Investigação em Ciências Orais e Biomédicas (UICOB), Faculdade de Medicina Dentária, Universidade de Lisboa, 1600-277 Lisboa, Portugal
Mariana Brito Da Cruz
Oral Biology and Biochemistry Research Group—Unidade de Investigação em Ciências Orais e Biomédicas (UICOB), Faculdade de Medicina Dentária, Universidade de Lisboa, 1600-277 Lisboa, Portugal
Gonçalo Garret
Department of Mechanical Engineering, Center for Microelectromechanical Systems (CMEMS), University of Minho, 4800-058 Guimarães, Portugal
Óscar Carvalho
Department of Mechanical Engineering, Center for Microelectromechanical Systems (CMEMS), University of Minho, 4800-058 Guimarães, Portugal
Filipe Silva
Department of Mechanical Engineering, Center for Microelectromechanical Systems (CMEMS), University of Minho, 4800-058 Guimarães, Portugal
António Mata
Oral Biology and Biochemistry Research Group—Unidade de Investigação em Ciências Orais e Biomédicas (UICOB), LIBPhys-FCT UIDB/04559/2020, Faculdade de Medicina Dentária, Universidade de Lisboa, 1600-277 Lisboa, Portugal
Helena Francisco
Grupo de Investigação Implantologia e Regeneração Óssea (UICOB), Faculdade de Medicina Dentária, Universidade de Lisboa, 1600-277 Lisboa, Portugal
Joana Faria Marques
Oral Biology and Biochemistry Research Group—Unidade de Investigação em Ciências Orais e Biomédicas (UICOB), Faculdade de Medicina Dentária, Universidade de Lisboa, 1600-277 Lisboa, Portugal
Peri-implantitis continues to be one of the major reasons for implant failure. We propose a new approach to the incorporation of MTA into zirconia implant surfaces with Nd:YAG laser and investigate the biological and the microbiological responses of peri-implant cells. Discs of zirconia stabilized with yttria and titanium were produced according to the following four study groups: Nd:YAG laser-textured zirconia coated with MTA (Zr MTA), Nd:YAG laser-textured zirconia (Zr textured), polished zirconia discs, and polished titanium discs (Zr and Ti). Surface roughness was evaluated by contact profilometry. Human osteoblasts (hFOB), gingival fibroblasts (HGF hTERT) and S. oralis were cultured on discs. Cell adhesion and morphology, cell differentiation markers and bacterial growth were evaluated. Zr textured roughness was significantly higher than all other groups. SEM images reveal cellular adhesion at 1 day in all samples in both cell lines. Osteoblasts viability was lower in the Zr MTA group, unlike fibroblasts viability, which was shown to be higher in the Zr MTA group compared with the Zr textured group at 3 and 7 days. Osteocalcin and IL-8 secretion by osteoblasts were higher in Zr MTA. The Zr textured group showed higher IL-8 values released by fibroblasts. No differences in S. oralis CFUs were observed between groups. The present study suggests that zirconia implant surfaces coated with MTA induced fibroblast proliferation and osteoblast differentiation; however, they did not present antibacterial properties.
