M-marker 2009, a marker set for mapping medaka mutants using PCR length polymorphisms with an automated microchip gel electrophoresis system

Abstract

We have developed a highly effective marker set for assigning medaka mutants to specific linkage groups (LG) in bulk segregation analysis by PCR product length polymorphisms (PLPs). The marker set permits analysis using an automated microchip electrophoresis system as well as conventional agarose gel electrophoresis.

Keywords

• positional cloning
• bulk segregation analysis
• automated electrophoresis systems
• agarose gel electrophoresis