Osteogenic Property Regulation of Stem Cells by a Hydroxyapatite 3D-Hybrid Scaffold With Cancellous Bone Structure

He Xia; Lun Dong; Min Hao; Yuan Wei; Jiazhi Duan; Xin Chen; Liyang Yu; Haijun Li; Yuanhua Sang; Hong Liu

doi:10.3389/fchem.2021.798299

Frontiers in Chemistry (Nov 2021)

Osteogenic Property Regulation of Stem Cells by a Hydroxyapatite 3D-Hybrid Scaffold With Cancellous Bone Structure

He Xia,
Lun Dong,
Min Hao,
Yuan Wei,
Jiazhi Duan,
Xin Chen,
Liyang Yu,
Haijun Li,
Yuanhua Sang,
Hong Liu

Affiliations

He Xia: State Key Laboratory of Crystal Materials, Shandong University, Jinan, China
Lun Dong: Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, China
Min Hao: State Key Laboratory of Crystal Materials, Shandong University, Jinan, China
Yuan Wei: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, China
Jiazhi Duan: State Key Laboratory of Crystal Materials, Shandong University, Jinan, China
Xin Chen: State Key Laboratory of Crystal Materials, Shandong University, Jinan, China
Liyang Yu: State Key Laboratory of Crystal Materials, Shandong University, Jinan, China
Haijun Li: Key Laboratory of Cardiovascular Proteomics of Shandong Province, Department of Geriatric Medicine, Qilu Hospital, Shandong University, Jinan, China
Yuanhua Sang: State Key Laboratory of Crystal Materials, Shandong University, Jinan, China
Hong Liu: State Key Laboratory of Crystal Materials, Shandong University, Jinan, China

DOI: https://doi.org/10.3389/fchem.2021.798299
Journal volume & issue: Vol. 9

Abstract

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Cancellous bone plays an indispensable role in the skeletal system due to its various functions and high porosity. In this work, chitosan and hydroxyapatite nanowires (CS@HAP NWs) hybrid nanostructured scaffolds with suitable mechanical properties, high porosity and a fine porous structure were prepared to simulate the 3-dimensional structure of cancellous bone. The 3D-hybrid scaffolds promote cell adhesion and the migration of human adipose-derived stem cells (hADSCs) inside the scaffolds. The cavities in the scaffolds provide space for the hADSCs proliferation and differentiation. Moreover, the various contents of HAP and the induced mechanical property changes regulate the differentiation of hADSCs toward osteoblasts. Overall, cellular fate regulation of hADSCs via rationally engineered HAP-based hybrid scaffolds is a facile and effective approach for bone tissue engineering.

Published in Frontiers in Chemistry

ISSN: 2296-2646 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: http://journal.frontiersin.org/journal/chemistry

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