Femtomolar detection of nucleic acid based on functionalized gold nanoparticles
Huang Jiaoqi,
Zhang Yang,
Lin Zhongquan,
Liu Wei,
Chen Xueping,
Liu Yu,
Tian Huiyan,
Liu Qiqian,
Gillibert Raymond,
Spadavecchia Jolanda,
Djaker Nadia,
de la Chapelle Marc Lamy,
Xiang Yang,
Fu Weiling
Affiliations
Huang Jiaoqi
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China
Zhang Yang
Department of Laboratory Medicine, Chongqing General Hospital, Chongqing 400038, China
Lin Zhongquan
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China
Liu Wei
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China
Chen Xueping
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China
Liu Yu
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China
Tian Huiyan
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China
Liu Qiqian
Université Paris 13, Sorbonne Paris Cité, UFR SMBH, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, F-93017 Bobigny, France
Gillibert Raymond
Université Paris 13, Sorbonne Paris Cité, UFR SMBH, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, F-93017 Bobigny, France
Spadavecchia Jolanda
Université Paris 13, Sorbonne Paris Cité, UFR SMBH, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, F-93017 Bobigny, France
Djaker Nadia
Université Paris 13, Sorbonne Paris Cité, UFR SMBH, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, F-93017 Bobigny, France
de la Chapelle Marc Lamy
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China
Xiang Yang
Department of Clinical Microbiology and Immunology, Faculty of Pharmacy and Medical Laboratory Sciences, Third Military Medical University (Army Medical University), Chongqing 400038, China
Fu Weiling
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), No. 29 Gaotanyan street, Shapingba, Chongqing 400038, China
Deoxyribonucleic acid (DNA) detection is essential for the accurate and early diagnosis of a disease. In this study, a femtomolar DNA detection method based on the exploitation of the localized surface plasmon (LSP) resonance of gold nanoparticles (AuNPs) was developed. We prepared Poly Ethylen Glycol (PEG) functionalized AuNPs with a specific DNA capture probe (CP) directly modified on the gold surface. Two strategies are proposed using different kinds of CP to detect the target DNA (tDNA). In the first strategy, CP is the complementary of the complete sequence of the DNA (CCP method). For the second strategy, we used two CPs, which were half complementary to tDNA, and these were hybridized with tDNA to form sandwich structures (MIX method). The results showed that our detection methods are highly sensitive and that the limits of detection of 124 am and 2.54 fm tDNA can be reached when using the CCP and MIX methods, respectively. In addition, the specificity of our two strategies is also demonstrated with mismatched DNAs. The proposed method provides a simple, fast, sensitive and specific DNA biosensor, which has the potential to be used for point-of-care tests (POCT).
