Encapsulation-Dependent Enhanced Emission of Near-Infrared Nanoparticles Using in vivo Three-Photon Fluorescence Imaging

Ye Du; Nuernisha Alifu; Zhiyuan Wu; Runze Chen; Xiaozhen Wang; Guang Ji; Qian Li; Jun Qian; Bin Xu; Dong Song

doi:10.3389/fbioe.2020.01029

Frontiers in Bioengineering and Biotechnology (Sep 2020)

Encapsulation-Dependent Enhanced Emission of Near-Infrared Nanoparticles Using in vivo Three-Photon Fluorescence Imaging

Ye Du,
Nuernisha Alifu,
Zhiyuan Wu,
Runze Chen,
Xiaozhen Wang,
Guang Ji,
Qian Li,
Jun Qian,
Bin Xu,
Dong Song

Affiliations

Ye Du: Department of Breast Surgery, The First Hospital, Jilin University, Changchun, China
Nuernisha Alifu: State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, JORCEP (Sino-Swedish Joint Research Center of Photonics), Zhejiang University, Hangzhou, China
Zhiyuan Wu: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, China
Runze Chen: State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, JORCEP (Sino-Swedish Joint Research Center of Photonics), Zhejiang University, Hangzhou, China
Xiaozhen Wang: Department of Breast Surgery, The First Hospital, Jilin University, Changchun, China
Guang Ji: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, China
Qian Li: Department of Breast Surgery, The First Hospital, Jilin University, Changchun, China
Jun Qian: State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, JORCEP (Sino-Swedish Joint Research Center of Photonics), Zhejiang University, Hangzhou, China
Bin Xu: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, China
Dong Song: Department of Breast Surgery, The First Hospital, Jilin University, Changchun, China

DOI: https://doi.org/10.3389/fbioe.2020.01029
Journal volume & issue: Vol. 8

Abstract

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We discovered a unique fluorescent enhancement of dye encapsulated polymeric nanoparticles, which strongly depended on the polymeric matrix. Interestingly, the polymer nanoparticles containing a NIR emissive dye exhibited remarkable enhancement of emission encapsulated by the polymer amphiphilic polymer containing polystyrene (PS) moiety, whereas the nanoparticles showed weak fluorescence when using other polymer encapsulation. The highest fluorescent quantum yield of nanoparticles can reach 27% by using PS-PEG encapsulation, where the strong NIR fluorescence can be observed. These ultra-bright fluorescence nanoparticles also possess a strong three-photon fluorescence and show a good candidate for in vivo vascular three-photon fluorescence imaging of mouse brain and ear under 1550 nm fs laser excitation. A fine three-dimensional (3D) reconstruction with an imaging depth of 635 and 180 μm was achieved, respectively. We further demonstrate that these nanoparticles can effectively target the sentinel lymph node (SLN) of mice.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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