TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity

Yongliang Zhang; Gaoyuan Song; Neeraj K. Lal; Ugrappa Nagalakshmi; Yuanyuan Li; Wenjie Zheng; Pin-jui Huang; Tess C. Branon; Alice Y. Ting; Justin W. Walley; Savithramma P. Dinesh-Kumar

doi:10.1038/s41467-019-11202-z

Nature Communications (Jul 2019)

TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity

Yongliang Zhang,
Gaoyuan Song,
Neeraj K. Lal,
Ugrappa Nagalakshmi,
Yuanyuan Li,
Wenjie Zheng,
Pin-jui Huang,
Tess C. Branon,
Alice Y. Ting,
Justin W. Walley,
Savithramma P. Dinesh-Kumar

Affiliations

Yongliang Zhang: Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis
Gaoyuan Song: Department of Plant Pathology and Microbiology, Iowa State University
Neeraj K. Lal: Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis
Ugrappa Nagalakshmi: Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis
Yuanyuan Li: Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis
Wenjie Zheng: Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis
Pin-jui Huang: Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis
Tess C. Branon: Departments of Genetics, Stanford University
Alice Y. Ting: Departments of Genetics, Stanford University
Justin W. Walley: Department of Plant Pathology and Microbiology, Iowa State University
Savithramma P. Dinesh-Kumar: Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis

DOI: https://doi.org/10.1038/s41467-019-11202-z
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 17

Abstract

Read online

Plant NLR receptors trigger immune signaling following recognition of pathogen effectors. Here, Zhang et al. optimize a TurboID-based proximity labeling approach and show that it can be used to identify interacting partners of N, an NLR that confers resistance to Tobacco mosaic virus.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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