Identification of Advantaged Genes for Lodging Resistance-Related Traits in the Temperate <i>geng</i> Group (<i>Oryza sativa</i> L.) Using a Genome-Wide Association Study
Laiyuan Zhai,
Duxiong Li,
Ningning Ren,
Shuangbing Zhu,
Dengji Wang,
Congcong Shen,
Kai Chen,
Jianlong Xu
Affiliations
Laiyuan Zhai
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Duxiong Li
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Ningning Ren
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Shuangbing Zhu
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Dengji Wang
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Congcong Shen
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Kai Chen
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Jianlong Xu
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
With the endless growth of the population and development of the economy and living standards, a high yield with good grain quality has become the prime objective of breeding. However, lodging is a major factor leading to a significant decline in rice (Oryza sativa L.) production and quality. We conducted genome-wide association analysis to detect quantitative trait loci (QTL)-related lodging resistance, using 395 temperate geng accessions. A total of 50 QTL affecting the six traits were detected, using 1,438,279 high-quality single nucleotide polymorphism markers. Five important QTL clusters were detected affecting the lodging resistance-related traits. The ten candidate genes were detected by performing gene differential expression analysis and haplotype analysis. Among them, LOC_Os07g48570 (OsDof-23) for qLA7.2, qSA7.2 and qPR7.3, LOC_Os08g29110 (wp2) for qLA8 and qSA8, and LOC_Os11g36440 (MHZ5) for qPR11 and qBR11.1 were considered the most likely candidate genes based on functional annotations. The results may facilitate the breeding of rice varieties resistant to lodging, to overcome the adverse effects of climate change.
