Methodological Aspects of the Modulus of Elastomeric Materials and Biological Cells by Force Spectroscopy Measurement

Abstract

In this article some methodological questions probe force spectroscopy at nanoindentation elastomeric materials discuss. They associated with the choice of the contact point and sliding the probe over the surface of the test material. An alternative method for choice of the contact point, in which the depth of probe's penetration into the sample becomes zero when using the model of Johnson-Kendall-Roberts proposes. It has been established that this point is located to the point of maximum adhesion force. According to nanoindentation data of erythrocyte by silicon probe with a radius of curvature of 60 nm and stiffness console 3 N/m is shown that the proposed version of the choice of the contact point provides a smaller variance the modulus of elasticity and access to a constant value of modulus of elasticity in the depth of penetration of 5-15 nm.

Keywords

• nanoindentation
• young's modulus
• contact point
• hertzian model
• johnson-kendall-roberts model
• force spectroscopy