Frontiers in Microbiology (Aug 2023)
Screening and genome-wide analysis of lignocellulose-degrading bacteria from humic soil
- Tianjiao Zhang,
- Tianjiao Zhang,
- Tianjiao Zhang,
- Tianjiao Zhang,
- Shuli Wei,
- Shuli Wei,
- Shuli Wei,
- Shuli Wei,
- Yajie Liu,
- Yajie Liu,
- Yajie Liu,
- Yajie Liu,
- Chao Cheng,
- Jie Ma,
- Jie Ma,
- Jie Ma,
- Jie Ma,
- Linfang Yue,
- Yanrong Gao,
- Yanrong Gao,
- Yanrong Gao,
- Yanrong Gao,
- Yuchen Cheng,
- Yuchen Cheng,
- Yuchen Cheng,
- Yongfeng Ren,
- Yongfeng Ren,
- Yongfeng Ren,
- Shaofeng Su,
- Shaofeng Su,
- Shaofeng Su,
- Shaofeng Su,
- Xiaoqing Zhao,
- Xiaoqing Zhao,
- Xiaoqing Zhao,
- Xiaoqing Zhao,
- Zhanyuan Lu,
- Zhanyuan Lu,
- Zhanyuan Lu,
- Zhanyuan Lu
Affiliations
- Tianjiao Zhang
- School of Life Science, Inner Mongolia University, Hohhot, China
- Tianjiao Zhang
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Tianjiao Zhang
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Tianjiao Zhang
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- Shuli Wei
- School of Life Science, Inner Mongolia University, Hohhot, China
- Shuli Wei
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Shuli Wei
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Shuli Wei
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- Yajie Liu
- School of Life Science, Inner Mongolia University, Hohhot, China
- Yajie Liu
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Yajie Liu
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Yajie Liu
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- Chao Cheng
- School of Life Science, Jining Normal University, Ulanqab, China
- Jie Ma
- School of Life Science, Inner Mongolia University, Hohhot, China
- Jie Ma
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Jie Ma
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Jie Ma
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- Linfang Yue
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Yanrong Gao
- School of Life Science, Inner Mongolia University, Hohhot, China
- Yanrong Gao
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Yanrong Gao
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Yanrong Gao
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- Yuchen Cheng
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Yuchen Cheng
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Yuchen Cheng
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- Yongfeng Ren
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Yongfeng Ren
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Yongfeng Ren
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- Shaofeng Su
- School of Life Science, Inner Mongolia University, Hohhot, China
- Shaofeng Su
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Shaofeng Su
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Shaofeng Su
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- Xiaoqing Zhao
- School of Life Science, Inner Mongolia University, Hohhot, China
- Xiaoqing Zhao
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Xiaoqing Zhao
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Xiaoqing Zhao
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- Zhanyuan Lu
- School of Life Science, Inner Mongolia University, Hohhot, China
- Zhanyuan Lu
- Inner Mongolia Academy of Agriculture and Husbandry Science, Hohhot, China
- Zhanyuan Lu
- Key Laboratory of Black Soil Protection And Utilization (Hohhot), Ministry of Agriculture and Rural Affairs, Hohhot, China
- Zhanyuan Lu
- Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, China
- DOI
- https://doi.org/10.3389/fmicb.2023.1167293
- Journal volume & issue
-
Vol. 14
Abstract
Crop straw contains huge amounts of exploitable energy, and efficient biomass degradation measures have attracted worldwide attention. Mining strains with high yields of cellulose-degrading enzymes is of great significance for developing clean energy and industrial production of related enzymes. In this study, we reported a high-quality genome sequence of Bacillus velezensis SSF6 strain using high-throughput sequencing technology (Illumina PE150 and PacBio) and assessed its lignocellulose degradation potential. The results demonstrated that the genome of B. velezensis SSF6 was 3.89 Mb and contained 4,015 genes, of which 2,972, 3,831 and 158 genes were annotated in the COGs (Clusters of Orthologous Groups), KEGG (Kyoto Encyclopedia of Genes and Genomes) and CAZyme (Carbohydrate-Active enZymes) databases, respectively, and contained a large number of genes related to carbohydrate metabolism. Furthermore, B. velezensis SSF6 has a high cellulose degradation capacity, with a filter paper assay (FPA) and an exoglucanase activity of 64.48 ± 0.28 and 78.59 ± 0.42 U/mL, respectively. Comparative genomic analysis depicted that B. velezensis SSF6 was richer in carbohydrate hydrolase gene. In conclusion, the cellulose-degrading ability of B. velezensis SSF6 was revealed by genome sequencing and the determination of cellulase activity, which laid a foundation for further cellulose degradation and bioconversion.
Keywords
- cellulose-degrading bacteria
- Bacillus velezensis
- whole-genome sequencing
- comparative genomic analysis
- carbohydrate-active enzyme
