SMG5-SMG7 authorize nonsense-mediated mRNA decay by enabling SMG6 endonucleolytic activity

Volker Boehm; Sabrina Kueckelmann; Jennifer V. Gerbracht; Sebastian Kallabis; Thiago Britto-Borges; Janine Altmüller; Marcus Krüger; Christoph Dieterich; Niels H. Gehring

doi:10.1038/s41467-021-24046-3

Nature Communications (Jun 2021)

SMG5-SMG7 authorize nonsense-mediated mRNA decay by enabling SMG6 endonucleolytic activity

Volker Boehm,
Sabrina Kueckelmann,
Jennifer V. Gerbracht,
Sebastian Kallabis,
Thiago Britto-Borges,
Janine Altmüller,
Marcus Krüger,
Christoph Dieterich,
Niels H. Gehring

Affiliations

Volker Boehm: Institute for Genetics, University of Cologne
Sabrina Kueckelmann: Institute for Genetics, University of Cologne
Jennifer V. Gerbracht: Institute for Genetics, University of Cologne
Sebastian Kallabis: CECAD Research Center, University of Cologne
Thiago Britto-Borges: Section of Bioinformatics and Systems Cardiology, Department of Internal Medicine III and Klaus Tschira Institute for Integrative Computational Cardiology, Heidelberg University Hospital
Janine Altmüller: Cologne Center for Genomics (CCG), University of Cologne
Marcus Krüger: Center for Molecular Medicine Cologne (CMMC), University of Cologne
Christoph Dieterich: Section of Bioinformatics and Systems Cardiology, Department of Internal Medicine III and Klaus Tschira Institute for Integrative Computational Cardiology, Heidelberg University Hospital
Niels H. Gehring: Institute for Genetics, University of Cologne

DOI: https://doi.org/10.1038/s41467-021-24046-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 19

Abstract

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Degradation of nonsense mediated mRNA decay (NMD) substrates is carried out by two seemingly independent pathways, SMG6-mediated endonucleolytic cleavage and/or SMG5-SMG7-induced accelerated deadenylation. Here the authors show that SMG5-SMG7 maintain NMD activity by permitting SMG6 activation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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