Revista Brasileira de Fruticultura (Mar 2021)

Self-incompatibility characterization in segregating populations of apple trees with DNA markers for S-alleles

  • Thyana Lays Brancher,
  • Maraisa Crestani Hawerroth,
  • Fernando José Hawerroth,
  • Marcus Vinícius Kvitschal,
  • Frederico Denardi,
  • Altamir Frederico Guidolin

DOI
https://doi.org/10.1590/0100-29452021167
Journal volume & issue
Vol. 43, no. 1

Abstract

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Abstract The objective of this study was to characterize the parents and respective populations of apple trees regarding S-alleles to confirm their genealogy and to evaluate the efficiency of the molecular markers used. Sixteen specific sets of primers were used for identification of apple S-alleles by PCR. Two segregating populations of the Epagri Apple Breeding Program resulting from crosses between ‘Fred Hough’ × ‘Monalisa’ and ‘M-11/00’ × ‘M-13/91’ were evaluated. The expected segregations are 1:1:1:1 for full compatibility and 1:1 for semi-compatibility, which can be confirmed by the X2 test. The ‘Fred Hough’ (S5S19) × ‘Monalisa’ (S2S10) cross proved to be fully compatible; and two triploids were identified among the hybrids as well. The ‘M-11/00’ (S3S19) × ‘M-13/91’ (S3S5) cross was characterized as semi-compatible based on DNA markers, and the segregation of the S-alleles in the hybrids was 1:1, as expected. The segregation of the DNA markers occurred together with their respective S-alleles: S2, S3, S5, S10, and S19. Thus, characterization of the S-alleles not only allowed identification of compatibility between parents but also identified contaminations in segregating populations.

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