PVA/pectin composite hydrogels inducing osteogenesis for bone regeneration

Ziwei Hu; Jianwen Cheng; Sheng Xu; Xiaojing Cheng; Jinmin Zhao; Zhi Wei Kenny Low; Pei Lin Chee; Zhenhui Lu; Li Zheng; Dan Kai

Materials Today Bio (Dec 2022)

PVA/pectin composite hydrogels inducing osteogenesis for bone regeneration

Ziwei Hu,
Jianwen Cheng,
Sheng Xu,
Xiaojing Cheng,
Jinmin Zhao,
Zhi Wei Kenny Low,
Pei Lin Chee,
Zhenhui Lu,
Li Zheng,
Dan Kai

Affiliations

Ziwei Hu: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Research Center for Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China
Jianwen Cheng: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Research Center for Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China
Sheng Xu: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Research Center for Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; School of Basic Medical Sciences, Guangxi Medical University, Nanning, 530021, China
Xiaojing Cheng: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Research Center for Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Life Science Institute, Guangxi Medical University, Nanning, 530021, China
Jinmin Zhao: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Research Center for Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China
Zhi Wei Kenny Low: Institute of Materials Research and Engineering (IMRE), A∗STAR, 2 Fusionopolis Way, #08-03 Innovis, 138634, Singapore
Pei Lin Chee: Institute of Materials Research and Engineering (IMRE), A∗STAR, 2 Fusionopolis Way, #08-03 Innovis, 138634, Singapore
Zhenhui Lu: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Research Center for Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Corresponding author. Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, 530021, China.
Li Zheng: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Research Center for Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China; Corresponding author. Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, 530021, China.
Dan Kai: Institute of Materials Research and Engineering (IMRE), A∗STAR, 2 Fusionopolis Way, #08-03 Innovis, 138634, Singapore; Institute of Sustainability for Chemicals, Energy and Environment (ISCE2), A∗STAR, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore; Corresponding author. Institute of Materials Research and Engineering (IMRE), A∗STAR, 2 Fusionopolis Way, #08-03 Innovis, 138634, Singapore.

Journal volume & issue: Vol. 16
p. 100431

Abstract

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Hydrogels composed from biomolecules have gained great interests as biomaterials for tissue engineering. However, their poor mechanical properties limit their application potential. Here, we synthesized a series of tough composite hydrogels from poly (vinyl alcohol) (PVA) and pectin for bone tissue engineering. With a balance of scaffold stiffness and pore size, PVA-Pec-10 hydrogel enhanced adhesion and proliferation of osteoblasts. The hydrogel significantly promoted osteogenesis in vitro by improving the alkaline phosphates (ALP) activity and calcium biomineralization, as well as upregulating the expressions of osteoblastic genes. The composite hydrogel also accelerated the bone healing process in vivo after transplantation into the femoral defect. Additionally, our study demonstrated that pectin and its Ca2+ crosslinking network play a crucial role of inducing osteogenesis through regulating the Ca2+/CaMKII and BMP-SMAD1/5 signaling. The optimized structure composition and multifunctional properties make PVA-Pec hydrogel highly promising to serve as a candidate for bone tissue regeneration.

Published in Materials Today Bio

ISSN: 2590-0064 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: https://www.journals.elsevier.com/materials-today-bio

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