Nature Communications (Jan 2017)
Massively parallel digital transcriptional profiling of single cells
- Grace X. Y. Zheng,
- Jessica M. Terry,
- Phillip Belgrader,
- Paul Ryvkin,
- Zachary W. Bent,
- Ryan Wilson,
- Solongo B. Ziraldo,
- Tobias D. Wheeler,
- Geoff P. McDermott,
- Junjie Zhu,
- Mark T. Gregory,
- Joe Shuga,
- Luz Montesclaros,
- Jason G. Underwood,
- Donald A. Masquelier,
- Stefanie Y. Nishimura,
- Michael Schnall-Levin,
- Paul W. Wyatt,
- Christopher M. Hindson,
- Rajiv Bharadwaj,
- Alexander Wong,
- Kevin D. Ness,
- Lan W. Beppu,
- H. Joachim Deeg,
- Christopher McFarland,
- Keith R. Loeb,
- William J. Valente,
- Nolan G. Ericson,
- Emily A. Stevens,
- Jerald P. Radich,
- Tarjei S. Mikkelsen,
- Benjamin J. Hindson,
- Jason H. Bielas
Affiliations
- Grace X. Y. Zheng
- 10x Genomics Inc.
- Jessica M. Terry
- 10x Genomics Inc.
- Phillip Belgrader
- 10x Genomics Inc.
- Paul Ryvkin
- 10x Genomics Inc.
- Zachary W. Bent
- 10x Genomics Inc.
- Ryan Wilson
- 10x Genomics Inc.
- Solongo B. Ziraldo
- 10x Genomics Inc.
- Tobias D. Wheeler
- 10x Genomics Inc.
- Geoff P. McDermott
- 10x Genomics Inc.
- Junjie Zhu
- 10x Genomics Inc.
- Mark T. Gregory
- Public Health Sciences Division, Translational Research Program, Fred Hutchinson Cancer Research Center
- Joe Shuga
- 10x Genomics Inc.
- Luz Montesclaros
- 10x Genomics Inc.
- Jason G. Underwood
- 10x Genomics Inc.
- Donald A. Masquelier
- 10x Genomics Inc.
- Stefanie Y. Nishimura
- 10x Genomics Inc.
- Michael Schnall-Levin
- 10x Genomics Inc.
- Paul W. Wyatt
- 10x Genomics Inc.
- Christopher M. Hindson
- 10x Genomics Inc.
- Rajiv Bharadwaj
- 10x Genomics Inc.
- Alexander Wong
- 10x Genomics Inc.
- Kevin D. Ness
- 10x Genomics Inc.
- Lan W. Beppu
- Clinical Research Division, Fred Hutchinson Cancer Research Center
- H. Joachim Deeg
- Clinical Research Division, Fred Hutchinson Cancer Research Center
- Christopher McFarland
- Seattle Cancer Care Alliance Clinical Immunogenetics Laboratory
- Keith R. Loeb
- Clinical Research Division, Fred Hutchinson Cancer Research Center
- William J. Valente
- Public Health Sciences Division, Translational Research Program, Fred Hutchinson Cancer Research Center
- Nolan G. Ericson
- Public Health Sciences Division, Translational Research Program, Fred Hutchinson Cancer Research Center
- Emily A. Stevens
- Clinical Research Division, Fred Hutchinson Cancer Research Center
- Jerald P. Radich
- Clinical Research Division, Fred Hutchinson Cancer Research Center
- Tarjei S. Mikkelsen
- 10x Genomics Inc.
- Benjamin J. Hindson
- 10x Genomics Inc.
- Jason H. Bielas
- Public Health Sciences Division, Translational Research Program, Fred Hutchinson Cancer Research Center
- DOI
- https://doi.org/10.1038/ncomms14049
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 12
Abstract
Single-cell gene expression analysis is challenging. This work describes a new droplet-based single cell RNA-seq platform capable of processing tens of thousands of cells across 8 independent samples in minutes, and demonstrates cellular subtypes and host–donor chimerism in transplant patients.