Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System

Diqing Su; Kai Wu; Venkatramana D. Krishna; Todd Klein; Jinming Liu; Yinglong Feng; Andres M. Perez; Maxim C.-J. Cheeran; Jian-Ping Wang; Jian-Ping Wang

doi:10.3389/fmicb.2019.01077

Frontiers in Microbiology (May 2019)

Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System

Diqing Su,
Kai Wu,
Venkatramana D. Krishna,
Todd Klein,
Jinming Liu,
Yinglong Feng,
Andres M. Perez,
Maxim C.-J. Cheeran,
Jian-Ping Wang,
Jian-Ping Wang

Affiliations

Diqing Su: Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, United States
Kai Wu: Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN, United States
Venkatramana D. Krishna: Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States
Todd Klein: Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN, United States
Jinming Liu: Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN, United States
Yinglong Feng: Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN, United States
Andres M. Perez: Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States
Maxim C.-J. Cheeran: Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States
Jian-Ping Wang: Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, United States
Jian-Ping Wang: Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN, United States

DOI: https://doi.org/10.3389/fmicb.2019.01077
Journal volume & issue: Vol. 10

Abstract

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The dissemination of Influenza A virus (IAV) throughout the world has become one of the main concerns for the health of both animals and human beings. An efficient and sensitive diagnostic tool is thus needed for the early detection of IAV. Here, we developed a wash-free magnetic bioassay and further integrated it with a handheld platform based on giant-magnetoresistance (GMR) sensors. The wash-free magnetic bioassay significantly accelerates and simplifies the detection process. This brand-new system was successful in detecting both IAV nucleoprotein and IAV-contained nasal swab samples from pigs on the farm. The limit of detection (LOD) is 0.3 nM for IAV nucleoprotein and 250 TCID50/mL for IAV-spiked nasal swab samples. The detection of nasal swab samples containing unpurified IAV was also performed, demonstrating the capability of the magnetic wash-free assay in the detection of biomarkers in complex sample matrix.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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