A study of antioxidant properties of Solen dactylus with DPPH, methods reducing power and TAC

Abstract

The purpose of this study was to evaluate the antioxidant power of knife shells, Solen dactylus using DPPH, reducing power, and TAC methods. Twenty shells were sampled at Ghouban Estuary in autumn and spring. 2 ml of pure methanol was added per 1 g of sample, and centrifuged at 11,000 rpm for 30 minutes. Then the supernatant was removed and transferred to the rotary machine for 5 minutes. In this method, 100 μl of the sample extract (0.5 g/l concentration) was added to 2 ml of DPPH (0.6 mm DPPH in 95% methanol) in a test tube and distilled into 3 ml distilled water. The reaction solution was then shaken vigorously and allowed to stand at room temperature in the dark for 30 minutes. The optical absorbance of the solution was measured in a wavelength of 517 nm using a spectrophotometer. In antioxidant studies, three different methods were used. DPPH, reducing power, and total antioxidants. Results of the DPPH method showed an average absorption of 67.79% between 2 seasons. In the absorption power reduction method, 62.2% was observed in autumn and 0.98 in spring. In the total antioxidant strength method, by comparing the absorbance of the extract solution with the absorption of ascorbic acid standards at concentrations of 50 to 400 mg/l, the total antioxidant in the autumn was 85.3 and 165.1 µg/ml in the spring.

Keywords

• razor-clam
• antioxidant
• ghouban estuary
• persian gulf