iScience (Apr 2023)
General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis
- Enqiang Chang,
- Yangyang Wang,
- Ruilou Zhu,
- Lingzhi Wu,
- Yitian Yang,
- Shuang Zeng,
- Ningtao Li,
- Xiaoguo Ruan,
- Mingyang Sun,
- Wei Zhang,
- Jun Zhou,
- Mengrong Miao,
- Hui Zhi,
- Hailin Zhao,
- Qian Chen,
- Qizhe Sun,
- Emer Chang,
- Albert Chang,
- Tingting Zhang,
- Xinfang He,
- Kan Liu,
- Songhua Ma,
- Weizhong Zhu,
- Youming Zhang,
- Luca Magnani,
- Daqing Ma,
- Jiaqiang Zhang
Affiliations
- Enqiang Chang
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China; Division of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UK
- Yangyang Wang
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Ruilou Zhu
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Lingzhi Wu
- Division of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UK
- Yitian Yang
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Shuang Zeng
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Ningtao Li
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Xiaoguo Ruan
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Mingyang Sun
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Wei Zhang
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Jun Zhou
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Mengrong Miao
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Hui Zhi
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Hailin Zhao
- Division of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UK
- Qian Chen
- Division of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UK
- Qizhe Sun
- Division of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UK
- Emer Chang
- Division of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UK
- Albert Chang
- Division of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UK
- Tingting Zhang
- Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China
- Xinfang He
- Department of Gynecology and Obstetrics, Henan Provincial People’s Hospital, Zhengzhou, Henan, China
- Kan Liu
- Department of Gynecology and Obstetrics, Henan Provincial People’s Hospital, Zhengzhou, Henan, China
- Songhua Ma
- Department of Physiology, School of Medicine, Nantong University, Nantong, Jiangsu, China
- Weizhong Zhu
- Department of Physiology, School of Medicine, Nantong University, Nantong, Jiangsu, China
- Youming Zhang
- Section of Genomic and Environmental Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London, UK
- Luca Magnani
- Division of Cancer, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London UK
- Daqing Ma
- Division of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UK; National Clinical Research Center for Child Health, Zhejiang, China; Corresponding author
- Jiaqiang Zhang
- Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China; Corresponding author
- Journal volume & issue
-
Vol. 26,
no. 4
p. 106534
Abstract
Summary: The cellular and molecular actions of general anesthetics to induce anesthesia state and also cellular signaling changes for subsequent potential “long term” effects remain largely elusive. General anesthetics were reported to act on voltage-gated ion channels and ligand-gated ion channels. Here we used single-cell RNA-sequencing complemented with whole-cell patch clamp and calcium transient techniques to examine the gene transcriptome and ion channels profiling of sevoflurane and propofol, both commonly used clinically, on the human fetal prefrontal cortex (PFC) mixed cell cultures. Both propofol and sevoflurane at clinically relevant dose/concentration promoted “microgliosis” but only sevoflurane decreased microglia transcriptional similarity. Propofol and sevoflurane each extensively but transiently (<2 h) altered transcriptome profiling across microglia, excitatory neurons, interneurons, astrocytes and oligodendrocyte progenitor cells. Utilizing scRNA-seq as a robust and high-through put tool, our work may provide a comprehensive blueprint for future mechanistic studies of general anesthetics in clinically relevant settings.
