3-Deoxysappanchalcone isolated from Caesalpinia sinensis shows anticancer effects on HeLa and PC3 cell lines: invasion, migration, cell cycle arrest, and signaling pathway
Dian Lv,
Qi Lai,
Qi Zhang,
Ji-hong Wang,
Yuan-ce Li,
Guang-Zhi Zeng,
Jun-Lin Yin
Affiliations
Dian Lv
Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education; School of Ethnic Medicine, Yunnan Minzu University, Kunming, China
Qi Lai
Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education; School of Ethnic Medicine, Yunnan Minzu University, Kunming, China
Qi Zhang
Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education; School of Ethnic Medicine, Yunnan Minzu University, Kunming, China
Ji-hong Wang
Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education; School of Ethnic Medicine, Yunnan Minzu University, Kunming, China
Yuan-ce Li
Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education; School of Ethnic Medicine, Yunnan Minzu University, Kunming, China
Guang-Zhi Zeng
Corresponding author.; Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education; School of Ethnic Medicine, Yunnan Minzu University, Kunming, China
Jun-Lin Yin
Corresponding author.; Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education; School of Ethnic Medicine, Yunnan Minzu University, Kunming, China
To study the antitumor activity of compound 3-desoxysulforaphane (3-DSC) isolated from Caesalpinia sinensis, SRB assay, clone formation assay, flow cytometric cell cycle assay, scratch assay, transwell assay, and molecular docking were used to investigate the inhibitory effect of 3-DSC on HeLa and PC3 cells. The results showed that 3-DSC inhibited the cell migration and invasion by down-regulating expression of N-cadherin, Vimentin, MMP-2, and MMP-9 in HeLa and PC3 cells; It also inhibits cell proliferation by promoting the expression of CDK1 (cyclin-dependent kinases 1) and CDK2 (cyclin-dependent kinases 2), which arrests the tumor cell cycle at G2 phase. 3-DSC inhibits phosphorylation of AKT and ERK and upregulates the expression of the tumor suppressor gene p53. Molecular docking results confirmed that 3-DSC could bind firmly to AKT. In conclusion, 3-DSC inhibited the proliferation, migration and invasion of HeLa and PC3 cells.
