A Rabbit Monoclonal Antibody against the Antiviral and Cancer Genomic DNA Mutating Enzyme APOBEC3B

William  L. Brown; Emily  K. Law; Prokopios  P. Argyris; Michael  A. Carpenter; Rena Levin-Klein; Alison  N. Ranum; Amy  M. Molan; Colleen  L. Forster; Brett  D. Anderson; Lela Lackey; Reuben  S. Harris

doi:10.3390/antib8030047

Antibodies (Sep 2019)

A Rabbit Monoclonal Antibody against the Antiviral and Cancer Genomic DNA Mutating Enzyme APOBEC3B

William L. Brown,
Emily K. Law,
Prokopios P. Argyris,
Michael A. Carpenter,
Rena Levin-Klein,
Alison N. Ranum,
Amy M. Molan,
Colleen L. Forster,
Brett D. Anderson,
Lela Lackey,
Reuben S. Harris

Affiliations

William L. Brown: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Emily K. Law: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Prokopios P. Argyris: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Michael A. Carpenter: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Rena Levin-Klein: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Alison N. Ranum: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Amy M. Molan: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Colleen L. Forster: Clinical and Translational Science Institute, University of Minnesota, Minneapolis, MN 55455, USA
Brett D. Anderson: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Lela Lackey: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Reuben S. Harris: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA

DOI: https://doi.org/10.3390/antib8030047
Journal volume & issue: Vol. 8, no. 3
p. 47

Abstract

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The DNA cytosine deaminase APOBEC3B (A3B) is normally an antiviral factor in the innate immune response. However, A3B has been implicated in cancer mutagenesis, particularly in solid tumors of the bladder, breast, cervix, head/neck, and lung. Here, we report data on the generation and characterization of a rabbit monoclonal antibody (mAb) for human A3B. One mAb, 5210-87-13, demonstrates utility in multiple applications, including ELISA, immunoblot, immunofluorescence microscopy, and immunohistochemistry. In head-to-head tests with commercial reagents, 5210-87-13 was the only rabbit monoclonal suitable for detecting native A3B and for immunohistochemical quantification of A3B in tumor tissues. This novel mAb has the potential to enable a wide range of fundamental and clinical studies on A3B in human biology and disease.

Published in Antibodies

ISSN: 2073-4468 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: http://www.mdpi.com/journal/antibodies

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