Frontiers in Bioengineering and Biotechnology (Sep 2022)
Genetic engineering of baculovirus-insect cell system to improve protein production
- Minqing Hong,
- Minqing Hong,
- Minqing Hong,
- Tingting Li,
- Tingting Li,
- Tingting Li,
- Wenhui Xue,
- Wenhui Xue,
- Wenhui Xue,
- Sibo Zhang,
- Sibo Zhang,
- Sibo Zhang,
- Lingyan Cui,
- Lingyan Cui,
- Lingyan Cui,
- Hong Wang,
- Hong Wang,
- Hong Wang,
- Yuyun Zhang,
- Yuyun Zhang,
- Yuyun Zhang,
- Lizhi Zhou,
- Lizhi Zhou,
- Lizhi Zhou,
- Ying Gu,
- Ying Gu,
- Ying Gu,
- Ningshao Xia,
- Ningshao Xia,
- Ningshao Xia,
- Ningshao Xia,
- Shaowei Li,
- Shaowei Li,
- Shaowei Li
Affiliations
- Minqing Hong
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Minqing Hong
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Minqing Hong
- Xiang An Biomedicine Laboratory, Xiamen, China
- Tingting Li
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Tingting Li
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Tingting Li
- Xiang An Biomedicine Laboratory, Xiamen, China
- Wenhui Xue
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Wenhui Xue
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Wenhui Xue
- Xiang An Biomedicine Laboratory, Xiamen, China
- Sibo Zhang
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Sibo Zhang
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Sibo Zhang
- Xiang An Biomedicine Laboratory, Xiamen, China
- Lingyan Cui
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Lingyan Cui
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Lingyan Cui
- Xiang An Biomedicine Laboratory, Xiamen, China
- Hong Wang
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Hong Wang
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Hong Wang
- Xiang An Biomedicine Laboratory, Xiamen, China
- Yuyun Zhang
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Yuyun Zhang
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Yuyun Zhang
- Xiang An Biomedicine Laboratory, Xiamen, China
- Lizhi Zhou
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Lizhi Zhou
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Lizhi Zhou
- Xiang An Biomedicine Laboratory, Xiamen, China
- Ying Gu
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Ying Gu
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Ying Gu
- Xiang An Biomedicine Laboratory, Xiamen, China
- Ningshao Xia
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Ningshao Xia
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Ningshao Xia
- Xiang An Biomedicine Laboratory, Xiamen, China
- Ningshao Xia
- The Research Unit of Frontier Technology of Structural Vaccinology of Chinese Academy of Medical Sciences, Xiamen, China
- Shaowei Li
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, China
- Shaowei Li
- National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China
- Shaowei Li
- Xiang An Biomedicine Laboratory, Xiamen, China
- DOI
- https://doi.org/10.3389/fbioe.2022.994743
- Journal volume & issue
-
Vol. 10
Abstract
The Baculovirus Expression Vector System (BEVS), a mature foreign protein expression platform, has been available for decades, and has been effectively used in vaccine production, gene therapy, and a host of other applications. To date, eleven BEVS-derived products have been approved for use, including four human vaccines [Cervarix against cervical cancer caused by human papillomavirus (HPV), Flublok and Flublok Quadrivalent against seasonal influenza, Nuvaxovid/Covovax against COVID-19], two human therapeutics [Provenge against prostate cancer and Glybera against hereditary lipoprotein lipase deficiency (LPLD)] and five veterinary vaccines (Porcilis Pesti, BAYOVAC CSF E2, Circumvent PCV, Ingelvac CircoFLEX and Porcilis PCV). The BEVS has many advantages, including high safety, ease of operation and adaptable for serum-free culture. It also produces properly folded proteins with correct post-translational modifications, and can accommodate multi-gene– or large gene insertions. However, there remain some challenges with this system, including unstable expression and reduced levels of protein glycosylation. As the demand for biotechnology increases, there has been a concomitant effort into optimizing yield, stability and protein glycosylation through genetic engineering and the manipulation of baculovirus vector and host cells. In this review, we summarize the strategies and technological advances of BEVS in recent years and explore how this will be used to inform the further development and application of this system.
Keywords
