Nature Communications (Jul 2021)
A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays
- Yan Ni,
- Bas J. H. M. Rosier,
- Eva A. van Aalen,
- Eva T. L. Hanckmann,
- Lieuwe Biewenga,
- Anna-Maria Makri Pistikou,
- Bart Timmermans,
- Chris Vu,
- Sophie Roos,
- Remco Arts,
- Wentao Li,
- Tom F. A. de Greef,
- Marcel M. G. J. van Borren,
- Frank J. M. van Kuppeveld,
- Berend-Jan Bosch,
- Maarten Merkx
Affiliations
- Yan Ni
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Bas J. H. M. Rosier
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Eva A. van Aalen
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Eva T. L. Hanckmann
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Lieuwe Biewenga
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Anna-Maria Makri Pistikou
- Institute for Complex Molecular Systems, Eindhoven University of Technology
- Bart Timmermans
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Chris Vu
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Sophie Roos
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Remco Arts
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Wentao Li
- Virology Section, Infectious Diseases and Immunology Division, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Tom F. A. de Greef
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- Marcel M. G. J. van Borren
- Department of Clinical Chemistry, Rijnstate Hospital
- Frank J. M. van Kuppeveld
- Virology Section, Infectious Diseases and Immunology Division, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Berend-Jan Bosch
- Virology Section, Infectious Diseases and Immunology Division, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
- Maarten Merkx
- Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology
- DOI
- https://doi.org/10.1038/s41467-021-24874-3
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 12
Abstract
Many current immunoassays require multiple washing, incubation and optimization steps. Here the authors present Ratiometric Plug-and-Play Immunodiagnostics (RAPPID), a generic assay platform that uses ratiometric bioluminescent detection to allow sandwich immunoassays to be performed directly in solution.
