Cell Biology Unit, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
Gregory Harms
Cell Biology Unit, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany; Departments of Biology and Physics, Wilkes University, Wilkes Barre, United States
Cell Biology Unit, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany; University Cancer Center Mainz, University Medical Center Mainz, Mainz, Germany
The actin cytoskeleton is tightly controlled by RhoGTPases, actin binding-proteins and nucleation-promoting factors to perform fundamental cellular functions. We have previously shown that ERK3, an atypical MAPK, controls IL-8 production and chemotaxis (Bogueka et al., 2020). Here, we show in human cells that ERK3 directly acts as a guanine nucleotide exchange factor for CDC42 and phosphorylates the ARP3 subunit of the ARP2/3 complex at S418 to promote filopodia formation and actin polymerization, respectively. Consistently, depletion of ERK3 prevented both basal and EGF-dependent RAC1 and CDC42 activation, maintenance of F-actin content, filopodia formation, and epithelial cell migration. Further, ERK3 protein bound directly to the purified ARP2/3 complex and augmented polymerization of actin in vitro. ERK3 kinase activity was required for the formation of actin-rich protrusions in mammalian cells. These findings unveil a fundamentally unique pathway employed by cells to control actin-dependent cellular functions.
