Characterization and antimicrobial properties of silver nanoparticules biosynthesized from cornelian cherry (Cornus mas L.)

Abstract

Nanoparticles produced by green synthesis has been increasingly gaining popularity, especially because they are eco-friendly and low cost. In the present article, silver nanoparticles (AgNPs) were synthesized from the extracts prepared using cornelian cherry (Cornus mas L.) at two different temperatures. The properties of obtained AgNPs were determined through UV-Vis spectroscopy, SEM, EDX, FTIR, and XRD analyses, and their antimicrobial effects on four pathogenic bacteria were investigated. The analysis results conducted using UV-spectrophotometry, SEM, EDX, FTIR, and XRD on AgNPs prepared from extracts obtained at two different temperatures (20 °C and 60 °C) were similar. The groups playing a role in nanoparticle formation were determined to be C=C, C=O, and C-O, and it was also concluded that the two different extraction temperatures had no significant effect on nanoparticle synthesis and characterization. The cherry extract's antimicrobial activity was effective against Staphylococcus aureus, Listeria monocytogenes, and Salmonella Typhi, while it didn’t show activity against Escherichia coli O157:H7. The AgNPs at concentration of 25 mg/mL created inhibition zones of 9 mm, 9 mm, and 7 mm for L.monocytogenes, S.aureus, and S.Typhi, respectively, at 20 °C. It was seen that 25 mg/mL AgNPs synthesized at 60 °C formed 9 mm and 8 mm inhibition zones in S.aureus and L.monocytogenes cultures, respectively, whereas they showed no inhibiting activity against S.Typhi, and E.coli O157:H7. It has been seen that 20 °C has ease of application in two different temperatures applied in the preparation of silver nanoparticles and is a good alternative to chemical methods.

Keywords

• silver nanoparticles
• antimicrobial effects
• cornelian cherry
• cornus mas l.
• green synthesis