Journal of Plant Development (Dec 2010)
TRICHODERMA VIRIDE PERS. – EXPERIMENTAL MODEL FOR BIOLOGICAL AND BIOTECHNOLOGICAL INVESTIGATIONS OF MYCROMYCETA WITH IMPORTANCE IN OBTAINING PLANT PROTECTION BIOPRODUCTS
Abstract
The technological process for obtaining plant protection bioproducts contains 2 main phases: (i) biomass biosynthesis of microorganisms in a culture medium, available for industrialization and (ii) biomass conditioning of microorganism, the antagonistic micromycetes, respectively. For this type of activities it is essential to establish biological development parameters: (i) the optimum composition of the liquid culture medium for development of the fungus under aerobiotic conditions and (ii) the optimal parameters of biosynthesis in the studied medium. The biomass biosynthesis technology is discontinuous, of cascade type, and develops several phases: (1) preparing of the laboratory inoculum, (2) preparing of the fungal pure culture in Erlenmeyer bottles, (3) industrial (simulated) multiplication in the aired and agitated liquid medium.This paper presents some experimental aspects referring to: 1 – Characterization of the biologically active T. viride isolates, establishing and verifying of their biological thresholds; 2 – Evaluation and experimental verifying of the mass multiplication ability of antagonistic T. viride fungi on the culture media in order to select the optimum industrial culture substrate (medium); 3 – Biochimical characterization of T. viride isolates by electrophoretic analysis of their protein profile; 4 – Evaluation of the T. viride biological activity of T. viride isolates against phytopathogenic fungi with high practical importance: Fusarium graminearum Schwabe (T. Gibberella zeae (Schwein.) Petch), F. culmorum (W. G. Sm.) Sacc., Pythium ultimum Trow, Botrytis cinerea Pers., Sclerotinia sclerotiorum (Lib.) de Bary, Alternaria spp. [A. alternata (Fr.) Keissl., Alternaria radicina Meier, Drechsler and E. D. Eddy (Stemphylium radicinum (Meier, Drechsler and E. D. Eddy) Neerg.)] etc.; 5 – Processing of technological scheme for obtaining plant protection preparates based on biologically active isolates of T. viride.
