Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9

Peiwu Qin; Mahmut Parlak; Cem Kuscu; Jigar Bandaria; Mustafa Mir; Karol Szlachta; Ritambhara Singh; Xavier Darzacq; Ahmet Yildiz; Mazhar Adli

doi:10.1038/ncomms14725

Nature Communications (Mar 2017)

Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9

Peiwu Qin,
Mahmut Parlak,
Cem Kuscu,
Jigar Bandaria,
Mustafa Mir,
Karol Szlachta,
Ritambhara Singh,
Xavier Darzacq,
Ahmet Yildiz,
Mazhar Adli

Affiliations

Peiwu Qin: Department of Physics, University of California at Berkeley
Mahmut Parlak: Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia
Cem Kuscu: Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia
Jigar Bandaria: Department of Physics, University of California at Berkeley
Mustafa Mir: Department of Molecular and Cell Biology, University of California at Berkeley
Karol Szlachta: Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia
Ritambhara Singh: Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia
Xavier Darzacq: Department of Molecular and Cell Biology, University of California at Berkeley
Ahmet Yildiz: Department of Physics, University of California at Berkeley
Mazhar Adli: Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia

DOI: https://doi.org/10.1038/ncomms14725
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 10

Abstract

Read online

CRISPR-Cas9 has been used to image repetitive loci in live cells to explore genome organization. Here the authors demonstrate the imaging of low-repeat regions with a single-guide RNA and the imaging of a non-repetitive region, allowing the tracking of transcriptionally active and inactive regions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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