Occlusion bodies purity of Nuclear Polyhedrosis Virusand product

Abstract

The efficacy in controllinginsect pest in thecrop of a virus biopesticide was significant depends on the concentration of occlusion bodies (OB) of NPV and dosage of product released in crop field after spraying which how to meet level from 9,0 x 1011 to 3,0 x 1012 OBs per hecta. By that, it is considered to be necessaryforOBs purity and qualification when making using formulation.The result of a study undertaken in 2017 showed that theOBs of Spodoptera lituraNPV could be purified by rotational use of two times of SDS and three times of pure water. By the way, the quantity of NPV’s OB could be 2,35 x 1010 OB/ml and their harvested percentage reached 83.93%. The result of study was alsoshowed that it was possible to formulate bioproduct under the wettable powder for a hectare with adosage of 500 gram, in which the concentration of 2,0 x 109 OBs per gram equivalence an using dosage of 1 x 1012 Obs per ha of thecrop. It was also indicated that the efficacy in controllingthe insect pest reached to 81.23% with aproduct without adding 5% Boric axit and 87,14% for the product mixtured with 5% of Boric axit in alaboratory experiment, to 81,13% and 82.92% (equivalent) in greenhouse condition at 7 days after spraying.

Keywords

• npv (nucleo polyhedrosis virus)
• ob (occlusion bodies)
• pured obs
• spodopteraliturainsect pest
• wettable powder