Mitochondrial mechanisms of apoptosis in case of experimental combined trauma of the chest and both thighs

Abstract

The objective of the study was to investigate the features of blood neutrophils’ apoptosis in case of combined trauma of the chest and both thighs in rats. Material and methods. The analysis of cell samples to determine reactive oxygen species was evaluated by the flow laser cytometry method, using 2.7-dichlorodihydrofluorescein diacetate. The percentage of neutrophils with low transmembrane mitochondrial potential and percentage of apoptotic neutrophils were evaluated by the flow laser cytometry method, using specific kits. Results. It was established the progressive, statistically significant increasing of Annexin V- positive cells percentage from the first day of the experiment, with the highest values within 7-14 days of observation. On the 28th day of experiment, the reduction of apoptotic white blood cells percentage by 7.7% than the findings on the 14th day was observed, but it remained 33.3% higher than the control. Conclusion. One of the important signaling pathways of apoptosis triggering in case of experimental combined trauma of the chest and both thighs is reactive oxygen species overproduction and disruption of the mitochondrial inner membrane, due to the decreasing transmembrane potential on 3-7 days of observation.

Keywords

• closed pneumothorax
• skeletal injury
• rats
• cell death