Metabolic engineering strategies for microbial utilization of methanol

Yamei Gan; Xin Meng; Cong Gao; Wei Song; Liming Liu; Xiulai Chen

Engineering Microbiology (Sep 2023)

Metabolic engineering strategies for microbial utilization of methanol

Yamei Gan,
Xin Meng,
Cong Gao,
Wei Song,
Liming Liu,
Xiulai Chen

Affiliations

Yamei Gan: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China
Xin Meng: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China
Cong Gao: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China
Wei Song: School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China
Liming Liu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China
Xiulai Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; Corresponding author.

Journal volume & issue: Vol. 3, no. 3
p. 100081

Abstract

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The increasing shortage of fossil resources and environmental pollution has renewed interest in the synthesis of value-added biochemicals from methanol. However, most of native or synthetic methylotrophs are unable to assimilate methanol at a sufficient rate to produce biochemicals. Thus, the performance of methylotrophs still needs to be optimized to meet the demands of industrial applications. In this review, we provide an in-depth discussion on the properties of natural and synthetic methylotrophs, and summarize the natural and synthetic methanol assimilation pathways. Further, we discuss metabolic engineering strategies for enabling microbial utilization of methanol for the bioproduction of value-added chemicals. Finally, we highlight the potential of microbial engineering for methanol assimilation and offer guidance for achieving a low-carbon footprint for the biosynthesis of chemicals.

Published in Engineering Microbiology

ISSN: 2667-3703 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Microbiology
Website: https://www.sciencedirect.com/journal/engineering-microbiology

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