SERUM LEVEL AND PRODUCTION OF CYTOKINES BY PBMC IN PATIENTS WITH ATOPIC DERMATITIS

Abstract

The рарег presents the results of measurements of cytokine levels in serum and conditioned medium of PBMC cultures from the patients with atopic dermatitis with the exacerbation and remission in comparison with the healthy donors. We have shown that the serum levels of the key cytokines IL-5 and IL-13, proinflammatory cytokines IL-1β and IL-6 and the main Th1 cytokine — IFNγ — were higher compared to healthy donors. In the conditioned media of peripheral blood mononuclear cells in contrast, we have found a significant decrease of the spontaneous secretion of key cytokines IL-10 and IL-17. We have shown that the stimulated secretion of IL-5 and IL-13, IL-12 and INF-γ is significantly reduced in comparison with the control level. Only IL-1β revealed a statistically significant higher level of stimulated secretion without exacerbation of atopic dermatitis. The contemporary therapy has no effect on cytokine production.

Keywords

• atopic dermatitis
• cytokines
• serum
• conditioned medium