Acute Hepatopancreatic Necrosis Disease (AHPPND) control in Whiteleg shrimp (Litopenaeus vannamei Boone) used inulin source from Dahlia uber (Dahlia variabilis Willd.)

Abstract

The effect of Inulin derived from Dahlia tubers as a functional diet on the growth of white legs shrimp was studied to see its impact on AHPND infection. The research was conducted in a fiber tank filled with 500 L of seawater sterilized with chlorine and stocked with 100 PL30 shrimps/container. Shrimp were treated with feed containing Inulin extract from dahlia tubers at a dose of 2.5%, 5.0%, and 5.0% commercial Inulin and compared with a control. Each treatment was given 2 replications. Shrimp were then challenged with Vibrio parahaemolyticus strain AHPND at a density of 106 CFU/mL. The live shrimp were then processed histopathologically to view the microanatomical gut structure (midgut). The results showed that the mean survival of the shrimp in the control and 2.5% Inulin treatment groups was 97.3 ± 1.15% and 97.3 ± 2.3%, respectively, and the 5% Inulin treatment and commercial Inulin were each 96.9 ± 2.7%. Histopathological observations on Inulin treatment of 2.5% dahlia tuber extract and commercial Inulin showed that shrimp intestine had more lactic acid bacteria abundance than control and 5% Inulin extract. In conclusion, shrimp treated with inulin can reduce the risk of shrimp being infected with AHPND.