Immature mDA neurons ameliorate motor deficits in a 6-OHDA Parkinson's disease mouse model and are functional after cryopreservation

Dominique Leitner; Mahesh Ramamoorthy; Marion Dejosez; Thomas P. Zwaka

Stem Cell Research (Dec 2019)

Immature mDA neurons ameliorate motor deficits in a 6-OHDA Parkinson's disease mouse model and are functional after cryopreservation

Dominique Leitner,
Mahesh Ramamoorthy,
Marion Dejosez,
Thomas P. Zwaka

Affiliations

Dominique Leitner: Department of Cell, Developmental, and Regenerative Biology, Mount Sinai Icahn School of Medicine, New York, NY 10029, United States; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Huffington Foundation Center for Cell-Based Research in Parkinson's Disease, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States
Mahesh Ramamoorthy: Department of Cell, Developmental, and Regenerative Biology, Mount Sinai Icahn School of Medicine, New York, NY 10029, United States; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Huffington Foundation Center for Cell-Based Research in Parkinson's Disease, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States
Marion Dejosez: Department of Cell, Developmental, and Regenerative Biology, Mount Sinai Icahn School of Medicine, New York, NY 10029, United States; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Huffington Foundation Center for Cell-Based Research in Parkinson's Disease, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States
Thomas P. Zwaka: Department of Cell, Developmental, and Regenerative Biology, Mount Sinai Icahn School of Medicine, New York, NY 10029, United States; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Huffington Foundation Center for Cell-Based Research in Parkinson's Disease, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Corresponding author at: Black Family Stem Cell Institute, One Gustave L. Levy Place, New York, NY 10028, United States.

Journal volume & issue: Vol. 41

Abstract

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Parkinson's disease is associated with the loss of dopaminergic neurons in the midbrain. Clinical studies investigating replacement of these neurons with in vitro-generated neurons are currently underway. However, this approach has been limited by difficulties in scaling up on-demand production of midbrain dopaminergic (mDA) neurons from pluripotent stem cells. Cryo-preservation may offer a solution, as it allows for banking of quality controlled mDA neurons. In this study, we tested different freezing conditions and found that optimal cryopreservation of immature human mDA neurons at an early differentiation time point was achieved in STEM-CELLBANKER medium using a controlled freezing program. Key words: Parkinson's disease, Cryopreservation, Midbrain dopaminergic neurons, Human induced pluripotent stem cells, 6-OHDA mouse model

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal