The Journal of Poultry Science (May 2007)

Z-Chromosome Specific Primers for Chicken-Quail Hybrid Blastoderm

  • Soichi Takagi,
  • Tamao Ono,
  • Akira Tsukada,
  • Yusuke Atsumi,
  • Shusei Mizushima,
  • Noboru Saito,
  • Kiyoshi Shimada

DOI
https://doi.org/10.2141/jpsa.44.209
Journal volume & issue
Vol. 44, no. 2
pp. 209 – 212

Abstract

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A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.

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