Química Nova (Jan 2008)

<title language="eng">Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method

  • Isabela da Costa César,
  • Fernão Castro Braga,
  • Cristina Duarte Vianna-Soares,
  • Elzíria de Aguiar Nunan,
  • Gérson Antônio Pianetti,
  • Ligia Maria Moreira-Campos

DOI
https://doi.org/10.1590/S0100-40422008000800003
Journal volume & issue
Vol. 31, no. 8
pp. 1933 – 1936

Abstract

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This paper describes the development and validation of an UV-Visible spectrophotometric method for quantitation of genistein and genistin in soy dry extracts, after reaction with aluminum chloride. The method showed to be linear (r²= 0.9999), precise (R.S.D. < 2%), accurate (recovery of 101.56%) and robust. Seven samples of soy dry extracts were analyzed by the spectrophotometric validated method and by RP-HPLC. Genistein concentrations determined by spectrophotometry (0.63% - 16.05%) were slightly higher than values obtained by HPLC analysis (0.40% - 12.79%); however, the results of both methods showed a strong correlation.

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